Application

Research CategoryNeuroscience

Research Sub CategoryNeurochemistry & Neurotrophins

This Anti-Nerve Growth Factor-β Antibody, pain is validated for use in ELISA, WB, IH, FUNC for the detection of Nerve Growth Factor-β.

Immunohistochemistry: 1-2 µg/mL (see suggested protocol).

Immunoblotting: 1-2 µg/mL

Bioassays (Inhibition of biological activity in vitro): 1-2 µg/mL

in vivo animal studies: use neat

ELISA: 1-2 µg/mL

Optimal working dilutions must be determined by the end user.

BIOLOGICAL ACTIVITY:

This antibody completely inhibits neuron survival and the outgrowth actions of mouse NGF in chicken dorsal root ganglion in vitro.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Mouse nerve growth factor-beta purified from salivary glands.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1119

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS. Contains no preservative. Reconstitute to desired volume with sterile distilled water. Suggested concentration 0.5-1.0 mg/mL.

Specificity

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human BDNF, NT3 and NT4 by ELISA.

Storage and Stability

Maintain at lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.